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Waters UPLC, UHPLC, and HPLC Column Selection and Mobile ...
Using a wide mobile-phase pH range is an effective approach to change compound selectivity. Increase selectivity for: - Acids (Peaks 3 and 6) - Bases (Peaks 1 and 4) Neutrals (Peaks 2 and 5) are largely unaffected by mobile-phase pH. The pH of the mobile phase has the greatest impact on analyte retention.
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