Welcome to the PulseNet/OutbreakNet Central Regional …

1 Welcome to the PulseNet/OutbreakNet Central Regional Meeting 12/11/2018 March 6-8, 2019 Doubletree Hotel Downtown Omaha, NE Wednesday, March 6, 2019 7:00 am 8:00 am Registration Joint Session 8:00 am - 8:30 am Welcome & Introductions DFWED Leadership Opening Remarks 8:30 am 10:00 am Session I: The Future is Here: PulseNet/OutbreakNet Transition to WGS Lessons Learned from an Outbreak (outbreak ideas: Salmonella Enteritidis in a MI hatchery or E. coli O157 in leafy greens) Communications placeholder Open discussion 10:00 am-10:30 am Break and Program Tables*Representatives will be present at program tables during scheduled breaks to perform live demonstrations, provide information, or answer questions on their respective topic or program.

2 2 10:30 am 12:00 Session II: The Future is Here: Managing New Workflows, Communication of Outbreak Data, and Data Integration- Panel Perspectives from high volume vs low volume states How do we handle (report and communicate?) multi-state matches with WGS? How does WGS handle common pathogens? Salmonella prioritization: (FoodNet perspectives-Practices to keep serotyping information with rollout of WGS and limited funding) Local cluster detection: From interpretation & reporting to communication Utility of Campylobacter for WGS: Puppy outbreak & MN Experience Open discussion 12:00 pm-1:30 pm Lunch On Your Own PulseNet Track OutbreakNet and Environmental Health Track 1:30pm 4:00 pm (Break at midway point) PulseNet Updates at Federal/State Levels Adapting Workflows for WGS: Where Are We Now and What s to Come?

3 Planning committee can provide a template for discussions: 1. Current Status/Updates from CDC 2. Does your workflow incorporate CIDTs (Pros, Cons, How to improve run)? 3. Does your laboratory workflow allow for a 7 working day TAT (Yes (How), No (Why), and improvements) 4. Aside for $$, are there other issues preventing your lab from conducting real-time surveillance (what support/ideas would be required to improve it)? 5. How is your laboratory prioritizing sequencing all Salmonella samples? Room/Discussion Setup 1:30pm 4:30 pm (Break at midway point) OutbreakNet Updates WGS Transition: Moving Forward 1. Prioritization for interviewing and updates from the WGS working groups a. Discussion on WGS for E. coli and Campylobacter 2.

4 Local cluster detection mock demonstration 3. Communications Surrounding WGS a. Communications to the public and industry 4. Open Discussion 3 Thursday, March 7, 2019 7:00 am 8:30 am Registration Sunrise Sessions (Up to 3) 7:30 am 8:15 am Arrange room into smaller work groups to discuss workflows and designated liaison from each group provide a summary of discussion to the room to facilitate a question session. 1. Each laboratory individually present their overall laboratory workflows to larger group Best practices for automation or making WGS efficient without automation, etc. 4:00 pm 4:30 pm Discussion & Wrap Up 4:30 pm Adjourn for Day 1 4:30 pm Adjourn for Day 1 Breakout Sessions (Up to 3) 4:30 pm 5:30 pm 4 PulseNet Track OutbreakNet and Environmental Health Track 8:30 am 12:00 pm (Break at Midway Point) Presentations and Discussions Guidance for Implementing & Conducting PN Surveillance Using WGS: What You Need to Know: IT/BioInformatic Needs Data handeling/storage Pilot labs experience with BioNumerics conversions: Tips & Tricks FSIS perspectives: Analytic methods for WGS 8:30 am 12:00 pm (Break at midway point) Outbreak Successes and Challenges: Part I Interesting and Local Outbreaks 1.

5 Salmonella Mbandaka in MI 2. Open discussion [Break] Outbreak Successes and Challenges: Part II Challenging Outbreaks 1. Salmonella Adelaide in pre-cut melon 2. Open discussion 12:00 pm 1:30 pm Lunch On Your Own 12:00 pm 1:30 pm Lunch On Your Own 1:30 pm 4:30 pm (Break at Midway Point) Presentations and Discussions NCBI pipelines Prioritization for WGS when you don t have funding to sequence everything Addressing TATs for WGS Addressing QA/QC: Looking Through the Magnifying Glass 1. Understanding QC metrics (Science/rational for metrics and developing thresholds 2. Adapting reference methods to WGS: CAP/CLIA/ISO 17025 Considerations & Lessons Learned 3. Updates from CDC PulseNet: Analysis certification timelines & addressing issues 1:30 pm 4:30 pm (Break at Midway Point) Outbreak Successes and Challenges: Part III Restaurant Outbreaks/Environmental Assessments Panel 1.)

6 Outbreaks that incorporate NEARS, EA, food code a. Salmonella Typhimurium in chicken salad 2. Recall processes and trace back at the local EH level 3. Exclusions 5 4:30 pm Adjourn for Day 2 4:30 pm Adjourn for Day 2 Friday, March 8, 2019 8:00 am -8:30 am Registration Joint Session 8:30 am 11:45 am (Break at midway point) ELC Panel Discussion ELC Update o Discussion from state, Regional , and high/low burden laboratories o Discussion from the Epi Perspective in high/low burden states The Future is Here: Where Are We Now with CIDTs? Follow up on CIDT Forum? Panel discussion: What has changed for states since 2017 Meeting? New insights from states on use/implementation of CIDTs locally Clinical lab based surveillance for Norovirus Panel o Discussions about new program involving states o MN and WI have piloted program and can share experiences Closing Remarks 11:30 am?

7 Evaluation and Adjourn Breakout Sessions (Up to 3) 4:30 pm 5:30 pm 1. Introduction to Epidemiology Terms & Concepts for Lab 6 Optional Trainings 12:00 pm 4:00 pm BioNumerics for Laboratorians Instructor: CDC PulseNet Staff 1:00 pm 5:00 pm SEDRIC Instructor: 1:00 pm 5:00 pm Workshop #3 Instructor: PROGRAM TABLES Wednesday, March 6, 2019 | 10:00 am - 10:30 am & 3:00 pm - 3:30 pm Program Table and Representatives Table #1 NARMS and NST Table #2 CoEs and CIFOR Table #3 Illumina Table #4 FSIS FoodNet, SEDRIC, Applied Math Table #5 RRT Table #6 NORS and NEARS Thursday, March 7, 2019 | 10:00 am 10:30 am and 3:00 pm 3.

8 30 pm Program Table and Representatives Table #1 NARMS and NST Table #2 CoEs and CIFOR Table #3 Illumina Table #4 FSIS FoodNet, SEDRIC, Applied Math Table #5 RRT Table #6 NORS and NEARS 7 Hosted By: Centers for Disease Control and Prevention, Division of Foodborne, Waterborne and Environmental Diseases United States Food and Drug Administration United Sates Department of Agriculture, Food Safety and Inspection Service Council of State and Territorial Epidemiologists National Environmental Health Association Association of Public Health Laboratories