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Basic Image Processing with FIJI/ImageJ

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Basic Image Processing with FIJI/ImageJ . Kristopher Kubow, , James Madison University CONTENTS (CLICK TO JUMP TO SECTION). 1 No matter what you are doing, READ THIS FIRST ........................................ ........................................ .. 1. 2 Downloading FIJI/ImageJ ........................................ ........................................ ....................................... 2. 3 Getting started ........................................ ........................................ ........................................ ............... 2. 4 Improving intensity visualization ........................................ ........................................ ........................... 3. 5 Working with multi-dimensional images opened as hyperstacks ........................................ ................. 4. 6 Shading correction ........................................ ........................................ ........................................ ......... 5. 7 Common modifications for preparing a figure.

microscopy facility director. • Except for ethically applied simple linear histogram (intensity) adjustments, reducing bit depth, and cropping, ALL image processing procedures MUST be disclosed in your Methods section and/or figure legend. 1.2 CONVENTIONS USED IN …

  Procedures, Microscopy

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