DNA Isolation Procedures

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DNA Isolation Procedures Michele K. Nishiguchi, Phaedra Doukakis, Mary Egan, David Kizirian, Aloysius Phillips, Lorenzo Prendini, Howard C. Rosenbaum, Elizabeth Torres, Yael Wyner, Rob DeSalle and Gonzalo Giribet Contents 1 Introduction ...... 250. 2 Materials ... 250. Organellar DNA .. .. .. 250. Plants and Algae .. .. .. 251. Microscopic Organisms ........................................ ................... 252. Solutions ........................................ ........... 253. 3 Methods .. ... 261. Kits ........................................ .......... 261. The Generic DNA Preparation ........................................ ........................... 261. 4 Protocols.

250 Michele K. Nishiguchi, Phaedra Doukakis, Mary Egan et al. Protocol 23: Isolation of poly(A)+ RNA: Selection of polyadenylated RNA from total RNA by

  Protocol