Flow Cytometry Basics Guide - Bio-Rad

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Flow CytometryPractical Advice to Get You Started in FlowFlow Cytometry Basics GuideVisit for more information Flow Cytometry Basics Guide | 1Table of ContentsChapter 1 Principles of the Flow CytometerFluidics System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3Optics and Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4Signal and Pulse Processing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6Electrostatic Cell Sorting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9Chapter 2 Principles of FluorescenceFluorophores and Light.

Fig. 1. Hydrodynamic focusing produces a single stream of particles. Without hydrodynamic focusing, the cuvette (typically 250 x 250 µm or 180 x 480 µm) or nozzle of the instrument (typically 70-130 µm) would not create a focused stream of cells and analysis of single cells would not be possible . With hydrodynamic focusing the cells flow in

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