Flow Cytometry Basics Guide - Bio-Rad

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Fig. 1. Hydrodynamic focusing produces a single stream of particles. Without hydrodynamic focusing, the cuvette (typically 250 x 250 µm or 180 x 480 µm) or nozzle of the instrument (typically 70-130 µm) would not create a focused stream of cells and analysis of single cells would not be possible . With hydrodynamic focusing the cells flow in

  Single