FlowJo Basic Tutorial - flowlab-childrens-harvard.com

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FlowJo Basic Tutorial FlowJo is software for the analysis of flow cytometry data. This Tutorial will quickly introduce you to FlowJo using an example experiment, where a FITC anti-CD8 reagent is titrated to determine the most economical concentration for staining in future experiments. Table of Contents Introduction ........................................ ........................................ ..................... 1. Step 1: Load samples into the workspace ........................................ ............... 3. Step 2: Analyze the control sample in detail ........................................ .......... 7. Step 3: Copy gates and statistics to all samples ........................................ ...... 12. Step 4: Verify gates on all samples.

Drag to draw a gate to set the range of fluorescence values that will define FL1 positive cells. Name this subset FL1+. Click the plus sign to add the name to the list for future use. Click OK. There are several gating tools for bivariate plots. Rectangle, Polygon, Quadrant or Ellipse gating tools can be selected from the top of the Graph

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