FlowJo Basic Tutorial - flowlab-childrens-harvard.com

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Drag to draw a gate to set the range of fluorescence values that will define FL1 positive cells. Name this subset FL1+. Click the plus sign to add the name to the list for future use. Click OK. There are several gating tools for bivariate plots. Rectangle, Polygon, Quadrant or Ellipse gating tools can be selected from the top of the Graph

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