FlowJo Basic Tutorial - flowlab-childrens-harvard.com

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FlowJo Basic Tutorial FlowJo is software for the analysis of flow cytometry data. This Tutorial will quickly introduce you to FlowJo using an example experiment, where a FITC anti-CD8 reagent is titrated to determine the most economical concentration for staining in future experiments. Table of Contents Introduction ........................................ ........................................ ..................... 1. Step 1: Load samples into the workspace ........................................ ............... 3. Step 2: Analyze the control sample in detail ........................................ .......... 7. Step 3: Copy gates and statistics to all samples ........................................ ...... 12. Step 4: Verify gates on all samples.

Human peripheral blood mononuclear cell preparation from a single healthy donor was divided into eight tubes. The first tube is the unstained control. The subsequent seven tubes were stained with a serial two-fold dilution of the anti-CD8-FITC reagent starting at 2 μg/ml. Cells were stained for 20 minutes, washed and resuspended in staining ...

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