FlowJo Basic Tutorial - flowlab-childrens-harvard.com

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Human peripheral blood mononuclear cell preparation from a single healthy donor was divided into eight tubes. The first tube is the unstained control. The subsequent seven tubes were stained with a serial two-fold dilution of the anti-CD8-FITC reagent starting at 2 μg/ml. Cells were stained for 20 minutes, washed and resuspended in staining ...

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