GC Large Volume Injection Optimization - US EPA

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Large Volume InjectionPresented ByTim AndersonGC Product ManagerPhenomenexEPA Region 6 QA ConferenceOctober, 2015GoalDevelop a method to use the least amount of sample and achieve the highest possible s/n response2Typical L Splitless500 g soilS/N = 25Larger L Splitless500 g soilS/N = 125Larger Injection / Smaller L Splitless100 g soilS/N = 25What to Watch Out For! L Dichloromethane @ 250 C & 14 psi = 336 LLiner Volume 990 L = 1008 L; exceeds liner Volume ! Flashback Ghost peaks Loss in sensitivity / linearity3PreliminaryAssumptions Pressure Pulsed Injection tend to improve analyte responses Improves transfer of analytes from the inlet into the column Started with Pressure Pulse and tested this assumption during throughout the experimentAnalytes: Started with a relatively simple list of Polycyclic Aroma

T im e - - > A b u n d a n c e T I C : L V I 0 0 3 . D T I C : 0 5 1 1 1 2 - M 0 1 . D T I C : L V I 0 0 4 . D T I C : L V I 0 0 6 . D Later Eluting PAH’s Pulsed Splitless for 0.6 min ... GC Large Volume Injection …

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