Gibson Assembly Cloning Guide, second edition

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Cloning GUIDE2ND EDITIONRESTRICTION DIGEST FREE, SEAMLESS CLONINGGibson Assembly Applications, tools, and protocols for the Gibson Assembly method: Single Insert Multiple Inserts Site-Directed Mutagenesis#DNAMY | (North America) or 1-858-228-4115 (outside North America) 2Contents Foreword Foreword The Gibson Assembly method has been an integral part of our work at Synthetic Genomics, Inc. and the J. Craig Venter Institute (JCVI) for nearly a decade, enabling us to synthesize a complete bacterial genome in 2008, create the first synthetic cell in 2010, and generate a minimal bacterial genome in 2016.

The Gibson Assembly® method is a cloning procedure that allows the cloning of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Instead, user-defined overlapping ends are incorporated into the fragments to allow the seamless joining of adjacent fragments.

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