Transcription of Gibson Assembly Cloning Guide, second edition
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Gibson Assembly Cloning Guide, second edition
Cloning GUIDE2ND EDITIONRESTRICTION DIGEST FREE, SEAMLESS CLONINGG ibson Assembly Applications, tools, and protocols for the Gibson Assembly method: Single Insert Multiple Inserts Site-Directed Mutagenesis#DNAMY | (North America) or 1-858-228-4115 (outside North America) 2 Contents Foreword Foreword The Gibson Assembly method has been an integral part of our work at Synthetic Genomics, Inc. and the J. Craig Venter Institute (JCVI) for nearly a decade, enabling us to synthesize a complete bacterial genome in 2008, create the first synthetic cell in 2010, and generate a minimal bacterial genome in 2016.
The Gibson Assembly® method is a cloning procedure that allows the cloning of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Instead, user-defined overlapping ends are incorporated into the fragments to allow the seamless joining of adjacent fragments.
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