HPLC Column Classification
hplc Column ClassificationBrian Bidlingmeyer,*Agilent;Chung Chow Chan,{Eli Lilly and Company;Patrick Fastino,{FDA;Richard Henry,*ThermoHypersil Keystone;Philip Koerner,*Phenomenex;Anne T. Maule,{3M Pharmaceuticals;Margareth Marques,* Pharmacopeia;Uwe Neue,*Waters;Linda Ng*USP Pharmaceutical Analysis 2 Expert Committee;Horacio Pappa, ;Lane Sander,*NIST;Carmen Santasania,*Supelco;Lloyd Snyder,{LC Resources;Timothy Wozniak,*USPPharmaceutical Analysis 2 Expert CommitteeABSTRACTThisStimuliarticle represents the conclusions and recommendations of the USP Working Group on HPLCColumns. The working group included the five largest manufacturers of hplc columns in the United States, along with theNational Institute of Standards and Technology (NIST) and USP. This work attempts to facilitate the selection of HPLCcolumns by the analyst when performing a USP L nomenclature to designate high-performance liquidchromatography ( hplc ) Column type was introduced forthe first time in theFourth SupplementtoUSP XIXin1978.}}}}
To compare columns on the same basis, the user should evaluate columnperformance byseparating the mixture iso-cratically using a mobile phase constituted by 80% metha-
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