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Microarray Analysis - The Basics

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Microarray AnalysisThe BasicsThomas GirkeDecember 9, 2011Microarray AnalysisSlide 1/42TechnologyChallengesData AnalysisData DepositoriesR and BioConductorHomework AssignmentMicroarray AnalysisSlide 2/42OutlineTechnologyChallengesData AnalysisData DepositoriesR and BioConductorHomework AssignmentMicroarray AnalysisTechnologySlide 3/42Microarray and Chip TechnologyDefinitionHybridization-based technique that allows simultaneousanalysis of thousands of samples on a solid ProfilingGene copy numberResequencingGenotypingSingle-nucle otide polymorphismDNA-protein interaction ( : ChIP-on-chip)Gene discovery ( : Tiling arrays)Identification of new cell technologiesProtein arraysCompound arraysMicroarray AnalysisTechnologySlide 4/42Why Microarrays?

Variations in biosamples, RNA quality and target labeling are normally the biggest noise introducing steps in array experiments. Careful experimental design and initial calibration experiments can minimize those challenges. Microarray Analysis Challenges Slide 12/42

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