MOLECULAR CHARACTERIZATION USING RAPD …

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, (4)2017: 658-666ISSN2278 9103658MOLECULAR CHARACTERIZATION USING RAPD AND SSR MARKERIN DIVERSE POMEGRANATE [PUNICA GRANATUM(L.)] GERMPLASMSaroj, , Parmar, ** Scholar, Department of Plant MOLECULAR Biology and Biotechnology,2**Advisor and Associate Professor, Collage of Agriculture,Tharad,Principal, Sardarkrushinagar Dantiwada Agricultural University, Saradrkrushinagar, Gujarat, Author (Punica granatumL.) belongs to the family Punicaceae exhibiting enormous morphological diversity is animportant horticultural crop of region of western India. We have inferred evolutionary relationship of 50 diverse genotypesusing RAPD and SSR markers. We have design new 14 SSR markers of which 3 were successful to depict polymorphicnature. Overall, 29 RAPD primers and eleven microsatellite (SSR) employed or MOLECULAR CHARACTERIZATION and screeningproduced a total 80/99 and 54/66 DNA fragments RAPD and SSR marker respectively.

RAPD and SSR marker in diverse pomegranate germplasm 659 and Doyle, 1987) with some modification. The quality and concentration of the extracted DNA were estimated by

  Germplasm