PCR Optimization Student Guide 2012 - Babec
PCR Optimization Table of Contents Fall 2012 Optimizing the polymerase chain Reaction Introduction ........................................ ........................................ ........................................ ..1 Review of Mathematics ........................................ ........................................ ...........................3 Solving Problems of Dilution and Concentration: Two Approaches ........................................ ..........4 Experiment Overview ........................................ ........................................ ............................7 Calculations Part 1: Overview of PCR Reaction Components ........................................ ........................8 Part 2: Varying the Magnesium Chloride Concentration.
PCR Optimization Student Guide Fall 2012 1 ! Optimizing the Polymerase Chain Reaction Frank H. Stephenson, Ph.D. Maria C. Abilock Applied Biosystems BABEC
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