Search results with tag "Bio rad laboratories"
1-800-4BIORAD Protein Assay Bio-Rad - Bio-Rad Laboratories
www.bio-rad.com1 Section 1 Introduction The Bio-Rad Protein Assay, based on the method of Bradford, is a simple and accurate procedure for determining concentration of solubi-
Buffer Formulations - Bio-Rad Laboratories
www.bio-rad.comBio-Rad Laboratories, Inc. 11-0864 1111 Sig 1211 Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699 Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
TIPS, TRICKS & BEST PRACTICES The Ultimate qPCR ... - Bio-Rad
www.bio-rad.com219 Bio-Rad Laboratories, Inc TIPS, TRICKS & BEST PRACTICES 9 Potential Real-Time PCR Pitfalls (and How to Avoid Them!) 1 USING DEGRADED RNA When it comes to qPCR, garbage in = garbage out. Degraded or contaminated RNA will yield low-quality
T100 Thermal Cycler - Bio-Rad
www.bio-rad.comT100™ Thermal Cycler Life Science Group Bulletin 6060 Rev C US/EG 13-2183 0114 Sig 1213 Bio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 3065 7550 Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
CHROMATOGRAPHY Aminex HPLC Columns - Bio-Rad
www.bio-rad.comLife Science Group Bulletin 6333 Rev A US/EG 12-1771 0113 Sig 1211 Bio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699 Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
CFX96 Touch Real-Time PCR System - Bio-Rad Laboratories
www.bio-rad.com1 | BIO-RAD LABORATORIES, INC. The CFX96 Touch Real-Time PCR System is a flexible and precise real-time PCR instrument. Its unsurpassed thermal cycler performance and innovative optical design produce accurate, reliable data. The powerful and intuitive software accelerates every step of your real-time PCR research, shortening the time
CFX Opus Real-Time PCR Systems - Bio-Rad Laboratories
www.bio-rad.comThe CFX Opus Real-Time PCR Systems are the next evolution in quantitative PCR (qPCR) from Bio-Rad Laboratories. With improved thermal performance and our proprietary, accurate optical shuttle system, your data will be more consistent. A sleek, modern design includes a refreshed and easy-to-use interface with more
Instruction Manual - Bio-Rad Laboratories
www.bio-rad.comBio-Plex Pro™ Diabetes Assays Instruction Manual For technical support, call your local Bio-Rad office, or in the U.S., call 1-800-424-6723. For research use only.
Transfection Methods Overview - Bio-Rad Laboratories
www.bio-rad.comwww.bio-rad.com/transfection Transfection Methods Overview 2 Overview Common Transfection Methods Reagent-Based Methods Lipids Calcium phosphate Cationic polymers
Product Information 2x Laemmli Sample 2x Laemmli Sample ...
www.bio-rad.comPremixed Buffers 161-0732 10x Tris/Glycine/SDS, 1 L 161-0772 10x Tris/Glycine/SDS, 5 L 161-0734 10x Tris/Glycine, 1 L 161-0771 10x Tris/Glycine, 5 L Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Dr., Hercules, CA 94547 USA 510-741-1000 4006028 Rev J. Introduction Bio-Rad’s Laemmli sample buffers are based on ...
Automated Droplet Generator - Bio-Rad Laboratories
www.bio-rad.comcontaining EvaGreen dye for use in real-time PCR, for research purposes only. Bio-Rad’s thermal cyclers and real-time thermal cyclers are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: …
Instruction Manual - Bio-Rad Laboratories
www.bio-rad.com† Note regarding Canadian EMC compliance: Le present appareil numerique n’emet pas de bruits radioelectrique depassant les limites applicables aux appareils numeriques de class A prescrites dans le reglement sur le brouillage radioelectrique edicte par …
Hematology and Coagulation Controls - Bio-Rad
www.bio-rad.comBio-Rad Laboratories QUALITY CONTROL SOLUTIONS FOR HEMATOLOGY AND COAGULATION Hematology and Coagulation Controls Your Quality Goals Realized
VARIANT II Hemoglobin - Bio-Rad
www.bio-rad.comBio-Rad Laboratories Hemoglobin TesTing Your patients count on you. You can count on the VARIANT ™ II System. Exceptional Ease of Use • Fully automated operation saves time and labor
フローサイトメトリー - Bio-Rad
www.bio-rad.comはじめに 本書はBio-Rad Laboratories 社発行の”Flow Cytometry Basic Guide”の日本語翻訳版です。 より理解を深めるため本書には原本にない章や記載が日本語版のみで追加されています。
CFX96 Dx System - Bio-Rad Laboratories
www.bio-rad.comCFX96 DxSystem Bulletin 7053 Ver C US/EG 20-0294 0420 Sig 0220 Website bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 00 800 00 24 67 23 Belgium 00 800 00 24 67 23 Brazil 4003 0399 Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 00 800 00 24 67 23 Denmark 00 800 00 24 67 23 Finland 00 800 00 24 67 23 ...
Transfer Buffer Formulations - Bio-Rad Laboratories
www.bio-rad.comBulletin 6211 Ver B US/EG 15-1379 1215 Sig 1215 Web site bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 43 1 877 89 01 177 Belgium 32 (0)3 710 53 00 Brazil 55 11 3065 7550 Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 45 44 52 10 00 Finland 358 09 804 22 00 France 33 01 47 95 69 65 Germany 49 89 31 884 0 …
Trans-Blot Turbo Transfer System - Bio-Rad Laboratories
www.bio-rad.comTrans-Blot Turbo System, transfer time is reduced to as little as 3 min, and the prepackaged transfer packs provide excellent transfer efficiency and reproducibility while eliminating the time and mess associated with traditional transfer methods. The Trans-Blot Turbo System consists of the main instrument with two blotting cassettes
Quick Start Bradford Protein Assay - Bio-Rad Laboratories
www.bio-rad.comcationic form (A max = 470 nm). However, when the dye binds to protein, it is converted to a stable unprotonated blue form (A max = 595 nm) (Reisner et al. 1975, Fazekes de St. Groth et al. 1963, Sedmack and Grossberg 1977). It is this blue protein-dye form that is detected at 595 nm in the assay using a spectrophotometer or microplate reader. H +H
Page 1/8 Safety Data Sheet - Bio-Rad Laboratories
www.bio-rad.comPage 2/8 Safety Data Sheet acc. to OSHA HCS Printing date 06/15/2015 Reviewed on 06/12/2015 Trade name: Ammonium Persulfate (Contd. of page 1) 40.1.5
Real-Time PCR Applications Guide - Bio-Rad Laboratories
www.bio-rad.com1.1.3 Hallmarks of an Optimized qPCR Assay Since real-time quantification is based on the relationship between initial template amount and the C T value obtained during amplification, an optimal qPCR assay is absolutely essential for accurate and reproducible quantification of your sample. The hallmarks of an optimized qPCR assay are:
Droplet Digital Applications Guide - Bio-Rad Laboratories
www.bio-rad.comQX100 ™ or QX200 Droplet Digital PCR system overcomes the previous lack of scalable and practical technologies for digital PCR implementation. 2oplet Digital PCR Applications Guide | Dr Droplet Digital PCR ddPCR has the following benefits for nucleic acid quantification:
Immun-Blot PVDF Membrane for Protein Blotting …
www.bio-rad.comBio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547 4006127 Rev A Immun-Blot® PVDF Membrane for Protein Blotting Instruction Manual 4006127.qxd 6/23/98 8:48 AM Page ii
High-Fidelity PCR Kit iProof - Bio-Rad Laboratories
www.bio-rad.comiProof™ High-Fidelity PCR Kit 2 units/µl, 25 µl 50U 172-5330 2 units/µl, 100 µl 200U 172-5331 For research purposes only Store at -20°C iProof is a high-fidelity DNA polymerase that offers extreme performance for all PCR
High-Fidelity DNA Polymerase iProof - Bio-Rad Laboratories
www.bio-rad.comStore iProof™ High-Fidelity DNA Polymerase at -20°C in a constant temperature freezer. When stored under these conditions, the polymerase is stable for one year after the ship date. Kit Contents Reagent 20U 100U 500U Description iProof Polymerase 10 µl 50 µl 250 µl iProof™ High Fidelity DNA Polymerase, 2 units/µl
Quick Guide - Bio-Rad Laboratories
www.bio-rad.comQuick Guide Activity 2: Determine the Effect of Temperature on the Reaction Rate 1. Label your cuvettes “0°C”, “22°C”, and “37°C”. 2.
Print EN 50419.tif (6 pages) - Bio-Rad Laboratories
www.bio-rad.comTitle: Print EN 50419.tif (6 pages) Author: schoetti Created Date: 8/24/2005 15:29:58
Nuvia Q Anion Exchange Resin - Bio-Rad Laboratories
www.bio-rad.com250 200 150 100 50 0 DB C BS A, 10 % B T, mg /m l 150 300 450 600 Linear velocity, cm/hr CHROMATOGRAPHY Nuvia™ Q Anion Exchange Resin sas l cn- Besi - t binding capacity at fast flow rates d Rapi mass transfer kinetics
Installation Qualification/Operational Qualification ...
www.bio-rad.comProtocol Acceptance by Customer and List of Tests Performed Bio-Rad Laboratories recommends that the IQ/OQ protocols be performed in total when the ... tool that allows the user to discriminate between assay and instrument problems. Fig. 1. The Experion system includes the following components: (1) automated electrophoresis
CFX96 Real-Time PCR Detection System - Bio-Rad Laboratories
www.bio-rad.comrunning a high-throughput laboratory and analyzing large gene expression studies. The CFX96 real-time PCR detection system builds on the power and flexibility of the C1000™ thermal cycler, adding an easy-to-install interchangeable reaction module to create an exceptional real-time PCR system. The system’s unsurpassed thermal performance plus
Protein Blotting Guide - Bio-Rad Laboratories
www.bio-rad.comprotein or protein-ligand interactions. This chapter provides an overview of the methods and workflow of protein blotting, which involves two phases: ... size, shape, and charge of the protein* and the pH, viscosity, and ionic strength of the transfer buffer, as well as gel composition also influence the elution of particular proteins from gels.
iScript cDNA Synthesis Kit - Bio-Rad Laboratories
www.bio-rad.comiScript cDNA Synthesis Kit provides a sensitive and easy-to-use solution for two-step reverse transcription quantitative all the reagents required for successful reverse transcription. The iScript Reverse Transcriptase is RNase H+, which provides greater sensitivity than RNase H– enzymes in …
04-0350 Mol Weight tn.FIN3 - Bio-Rad Laboratories
www.bio-rad.comthe data points that lie beyond the linear range, as shown in Figure 3. In Figure 3B, the values for the five largest proteins were omitted from the calculations, increasing the r2 value from 0.913 to 0.997. Most scientific calculators and software can generate the r2 value and the equation of the best-fit line from the data points.
Instruction Manual - Bio-Rad Laboratories
www.bio-rad.comcalibration standard for gel filtration/size exclusion chromatography (SEC) columns used in protein purification and analysis under nondenaturing conditions. It is appropriate for gels with exclusion limits approximately 60,000 to 5,000,000 Da. The mixture contains thyroglobulin, g-globulin, ovalbumin, myoglobin, and vitamin B12.
00-305/1156 acryl.poly tn - Bio-Rad Laboratories
www.bio-rad.comcause artifacts such as aberrant relative mobility, precipitation of some proteins and nucleic acids, streaking or smearing of bands, and run-to-run irreproducibility. In acrylamide, acrylic acid should be below 0.001% (w/w). This is determined by direct titration, and supported by both conductivity and pH measurement. 2.
Ethidium Bromide Solution, 10 mg/ml - Bio-Rad Laboratories
www.bio-rad.comEvitare l’esposizione - procurarsi speciali istruzioni prima dell’uso. 4006020B.qxd 09/19/2001 4:32 PM Page 6. 8 Section 4 Ordering Information Catalog Number Description 161-0433 Ethidium Bromide Solution, 10 mg/ml 161-0430 Ethidium Bromide Tablets, 10 x 10 mg
Image Lab Software User Guide - Bio-Rad Laboratories
www.bio-rad.comTableofContents Report 99 Chapter6AnalyzingImages 102 ImageTypes 102 AutoAnalysis 102 AutoDetectionSettings 103 MolecularWeightAnalysisSettings 104
DC Protein Assay Instruction Manual - Bio-Rad Laboratories
www.bio-rad.comVortex. 5. Add 4.0 ml reagent B into each test tube and vortex immediately. 6. After 15 minutes, absorbances can be read at 750 nm. The absorbances will be stable at least 1 hour. (See Troubleshooting Guide for recommendation on using a wavelength other than 750 nm.) 5.2 Microplate Assay Protocol 1. Preparation of working reagent
InstaGeneTM Matrix - Bio-Rad Laboratories
www.bio-rad.com3 2.2 DNA Preparation From Cultured Mammalian Cells 1 Pellet 200 µl of cell suspension from media in a microfuge tube. Spin at 10,000–12,000 rpm for 1 minute and remove the supernatant. 2 Resuspend cells in 1 ml of 1x PBS and centrifuge for 1 minute at 10,000–12,000 rpm. 3 Resuspend cells in autoclaved water at 20–30 cells/µl. 4 Add 20 µl of this cell suspension to 200 µl of ...
Gel Doc XR ChemiDoc XRS - Bio-Rad Laboratories
www.bio-rad.comThis software will run under Windows 2000 or XP, or on a Macintosh PowerPC. The amount of computer memory required for using the program is mainly determined by the size of the images you will scan and analyze. Images scanned at high resolution can be quite large. For this reason, we recommend that you archive images on a network file server or
QuantaSoft Analysis Pro Software - Bio-Rad Laboratories
www.bio-rad.comand assay strategies for Droplet Digital PCR (ddPCR). QuantaSoft AP is for use with data files generated on the QX100 or QX200 Droplet Reader using QuantaSoft Standard Edition …
CFX96 Touch Real-Time PCR System - Bio-Rad Laboratories
www.bio-rad.comexceptional quantitative PCR (qPCR) results in less than 30 minutes. Tenfold serial dilutions of 10 nanograms to 100 attograms cDNA from human spleen were used in each 20 μl reaction to detect 18S rRNA. 18S rRNA efficiency = 101.8%, r = 0.997. Total qPCR run time = 29 min. RFU, relative fluorescence units. Excellent uniformity.
Mini-PROTEAN Tetra Cell - Bio-Rad Laboratories
www.bio-rad.comMini-PROTEAN ® Tetra Cell. Instruction Manual. Catalog Numbers. 165-8000 165-8004 165-8001 165-8005 . 165-8002 165-8006 165-8003 165-8007
Real-Time PCR Applications Guide - Bio-Rad Laboratories
www.bio-rad.com7.2 DNA Extraction and Sample Preparation for GMO Detection 77 7.3 GM Soy Detection Using a Singleplex SYBR Green I qPCR Assay 78 7.3.1 Reaction Components 78 7.3.2 Cycling Protocol 79 7.3.3 Data Analysis 79 7.4 GM Soy Detection Using a Multiplex TaqMan qPCR Assay 81 7.4.1 Reaction Components 82 7.4.2 Cycling Protocol 82 7.4.3 Data Analysis 82 8.
Mini-PROTEAN Electrophoresis System - Bio-Rad Laboratories
www.bio-rad.comfor proteins in the 10–250 kD molecular weight range and can be used as a screening gel. Any kD Gel delivers exceptional resolution for proteins in the 10–100 kD range, which are typically represented in a 2-D sample, and hence is an ideal precast gel for 2-D applications.
BioLogic LP System - Bio-Rad Laboratories
www.bio-rad.comAdvantages of the BioLogic LP Data View Software Automatically collects UV and conductivity data from the BioLogic LP system using any PC running Windows XP or Windows 7 All three axes — UV, conductivity, and run time — can be rescaled independently both during and after
Bio-Plex Cytokine Assay - Bio-Rad Laboratories
www.bio-rad.comSection 7 Mixing Multiplex Assays: Bead, Standard, ... Both techniques are time consuming and can facilitate the analysis of only a single cytokine at a time. The Bio-Plex suspension array system, ... By multiplexing, it is possible to quantitate the level of multiple cytokines
Bio-Rad Laboratories
www.bio-rad.comoffering unrivaled precision and absolute quantification of target dna or rna molecules, our unique droplet digital pcr (ddpcr™) technology is leading to breakthroughs in such …
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