Transcription of Transfer Buffer Formulations - Bio-Rad Laboratories
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Transfer Buffer Formulations - Bio-Rad Laboratories
Transfer Buffer Formulations Protocol bulletin 6211. Transfer Buffer Formulations Towbin Buffer , 1 L. 25 mM Tris, 192 mM glycine, 20% (v/v) methanol (pH ) (catalog #1610734, The following buffers are recommended for use with all of without methanol, 1 L, 10 ). Bio-Rad 's electrophoretic Transfer cells. Care should be Tris base g taken when preparing these buffers because incorrect Glycine g formulation can result in a current that exceeds the diH2O 500 ml recommended conditions. Methanol 200 ml Adjust volume to 1 L with diH2O. TIPS The pH will range from pH to depending on the quality of the Tris, glycine, methanol, and diH2O. Use only high-quality, analytical grade methanol. Towbin Buffer with SDS, 1 L. Impure methanol can increase Transfer Buffer conductivity 25 mM Tris, 192 mM glycine, 20% (v/v) methanol, SDS (pH ). and yield a poor Transfer . Add 10 ml 10% SDS to 1 L Buffer prepared above.
Bulletin 6211 Ver B US/EG 15-1379 1215 Sig 1215 Web site bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 43 1 877 89 01 177 Belgium 32 (0)3 710 53 00 Brazil 55 11 3065 7550 Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 45 44 52 10 00 Finland 358 09 804 22 00 France 33 01 47 95 69 65 Germany 49 89 31 884 0 …
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