Transcription of Immun-Blot PVDF Membrane for Protein Blotting …
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Immun-Blot PVDF Membrane for Protein Blotting …
Bio-Rad laboratories , 2000 Alfred Nobel Dr., Hercules, CA 945474006127 Rev AImmun-Blot PVDF Membrane forProtein BlottingInstruction 6/23/98 8:48 AM Page iiSection 2 Membrane 3 Dot Blotting ..5 Section 4 Electrophoretic Blotting ..6 Section 5 Optimization of 6 References ..10 Section 7 Product Information ..11 Table of 6/23/98 8:48 AM Page iSection 1 IntroductionPVDF (polyvinylidene difluoride) mem-brane was originally introduced to Protein use asan ideal medium for the harsh chemical environ-ment of N-terminal, or Edman degradation,sequencing. The hydrophobicity of PVDF makes it an ideal support for binding proteins inelectrophoretic and dot Blotting of the hydrophobic nature of PVDF, itdoes require a prewetting step in alcohol.
Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547 4006127 Rev A Immun-Blot® PVDF Membrane for Protein Blotting Instruction Manual 4006127.qxd 6/23/98 8:48 AM Page ii
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