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Autophagy - Abcam

AutophagyTools for cell death series 23 ContentsIntroduction to cell death ..3 Autophagy ..3 -Types of Autophagy ..4 Macroautophagy ..4 Microautophagy ..4 Chaperone mediated Autophagy ..4 Autophagy : cellular and molecular mechanisms ..6 -Induction and phagophore formation ..8 -Phagophore elongation and autophagosome formation ..10 -Fusion, degradation and recycling ..12 - Autophagy regulation ..14 Autophagy and disease -Cancer ..14 -Neurodegenerative diseases ..15 -Cardiovascular diseases ..15 -Infectious disease ..16 Autophagy detection -Markers of Autophagy ..17 -Visual identification ..19 -ATG8/LC3 detection ..20 Western Blotting ..20 Turnover of LC3 II/ATG8 PE ..22 Luminescence based assays ..25 GFP ATG8/LC3 detection ..25 Fluorescence microscopy ..26 Tandem mRFP/mcherry GFP fluorescence microscopy ..28 Flow and multispectral imaging cytometry.

5 Autophagy Autophagy (from the Greek “auto”–self and “phagein”–to eat) is a general term for processes by which dysfunctional cellular components are …

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