Search results with tag "Dna extraction"
Banana DNA Extraction Lab - Mrs. Kornelsen's Classroom
akornelsen.weebly.comBanana DNA Extraction Lab Name: _____ Objectives: Describe where DNA is located in a plant cell. Explain what procedures are required to release DNA from a plant cell Observe the extraction of Genomic DNA from plant cells. Full of DNA What can we tell about the molecular structure of DNA by studying the characteristics of DNA
STRAWBERRY DNA EXTRACTION - Noble
www.noble.orgIn this activity, students will learn the simple method of DNA extraction, be able to explain the rationale of each step, and use simple household chemicals to extract DNA from a strawberry. DNA extraction is a fairly simple process. The first step is to create the lysis buffer. Lysis is a Greek word that means to break open.
EXTRACTING DNA FROM STRAWBERRIES - Michigan
www.michigan.govcompanies. For DNA extraction, detergent is used to lyse the cell so that DNA is released into the solution. Then alcohol is added to cause the DNA to precipitate out. Strawberries are chosen for this particular experiment because strawberry cell has eight copies of the genome, and it would be easier to extract the DNA since it is
Quick-DNA™ Miniprep Plus Kit - Zymo Research
files.zymoresearch.comApr 16, 2021 · The Quick-DNA™ Miniprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, plasmid, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin™ Technology allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 35 µl.
Whole Exome Sequencing and Analysis
www.nisc.nih.govOct 22, 2018 · We need a minimum of 150 ng of highly-purified genomic DNA (0.5 μg preferred) in a volume of 50 μl or less for WES. Samples should be submitted in 1.5-1.7 ml ... and the DNA extraction protocol used. Some damage can be repaired, but not all. For instance, abasic positions cannot be restored. As with all DNA samples, FFPE-derived
Home or hospital birth: the neonatal microbiota perspective
www.thelancet.comJan 12, 2022 · swabs were collected. Following DNA extraction, bacterial colonisation was assessed by profiling the 16S rRNA using Illumina MiSeq technology and applying bioinformatics pipelines, as previously described. 3. We did not observe significant . differences in colonisation diversity between those born at home and those born at the hospital, which was
Plant Genomic DNA Extraction by CTAB 2 Fiona
agriculture.uq.edu.auCTAB buffer 100ml 2.0 g CTAB (Hexadecyl trimethyl-ammonium bromide) 10.0 ml 1 M Tris pH 8.0 4.0 ml 0.5 M EDTA pH 8.0 (EthylenediaminetetraAcetic acid Di-sodium salt) 28.0 ml 5 M NaCl 40.0 ml H2O 1 g PVP 40 (polyvinyl pyrrolidone (vinylpyrrolidine homopolymer) Mw 40,000) Adjust all to pH 5.0 with HCL and make up to 100 ml with H2O. 1 M Tris pH 8.0
Real-Time PCR Applications Guide - Bio-Rad Laboratories
www.bio-rad.com7.2 DNA Extraction and Sample Preparation for GMO Detection 77 7.3 GM Soy Detection Using a Singleplex SYBR Green I qPCR Assay 78 7.3.1 Reaction Components 78 7.3.2 Cycling Protocol 79 7.3.3 Data Analysis 79 7.4 GM Soy Detection Using a Multiplex TaqMan qPCR Assay 81 7.4.1 Reaction Components 82 7.4.2 Cycling Protocol 82 7.4.3 Data Analysis 82 8.
DNA Extraction from Strawberry - University of California ...
pbge.faculty.ucdavis.eduWhere can DNA be found in the cell? What is DNA? What are the three basic steps in any DNA extraction? What is the purpose of the detergent? Of the sodium chloride? Of the alcohol? Describe the appearance of your final product. Is this a pure DNA sample? List some practical applications where extracting DNA is necessary.
DNA extraction protocol (Ben-Updated) - stccmop.org
www.stccmop.orgDNA Extraction Center for Coastal Margin Observation & Prediction (www.stccmop.org) Solutions: DNA extraction buffer: Contains 0.1 M EDTA @ pH 8, 1% SDS and 200 µg/mL proteinase K. Make a stock of 50 mL 0.1 M EDTA-1% SDS by combining 10 mL EDTA pH8, 5 mL 10% SDS and 35 mL MilliQ water for a total volume of 50
DNA Extraction Lab - Towson University
www.towson.eduDNA extraction and to avoid violent shaking or mixing that would shear the DNA. The process of isolating DNA requires that it be released from a cell whether it is a plant (which has extra protection with a cell wall), animal, fungi, or bacterium.
DNA Extraction from Strawberrie - ucbiotech.org
ucbiotech.org10 mLs DNA extraction buffer (soapy, salty wa ter) 15 mLs ice cold ethanol in test tube Procedure: Working together in groups of two, read through the entire procedure and follow the steps in Parts I, II and III. Part I - Preparing the DNA extract 1. Have one partner get the lab box while the other partner gets the strawberry and removes
DNA EXTRACTION METHODS - Open University of Sri Lanka
www.ou.ac.lkNon-Organic DNA Extraction Procedure 1. Cell Lysis Buffer - lyse cell membrane, nuclei are intact, pellet nuclei. 2. Resuspend nuclei in Protein Lysis Buffer containing a high concentration of Proteinase K. Lyse nuclear membrane and digest protein at 65oC for 2 hours. Temperature helps denature proteins, and Proteinase K auto digests itself 3.