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DNA extraction protocol (Ben-Updated) - stccmop.org

DNA Extraction Center for Coastal Margin Observation & Prediction (www.stccmop.org) Solutions: DNA extraction buffer: Contains 0.1 M EDTA @ pH 8, 1% SDS and 200 µg/mL proteinase K. Make a stock of 50 mL 0.1 M EDTA-1% SDS by combining 10 mL EDTA pH8, 5 mL 10% SDS and 35 mL MilliQ water for a total volume of 50

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STRAWBERRY DNA EXTRACTION - Noble

www.noble.org

In this activity, students will learn the simple method of DNA extraction, be able to explain the rationale of each step, and use simple household chemicals to extract DNA from a strawberry. DNA extraction is a fairly simple process. The first step is to create the lysis buffer. Lysis is a Greek word that means to break open.

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Banana DNA Extraction Lab - Mrs. Kornelsen's Classroom

akornelsen.weebly.com

Banana DNA Extraction Lab Name: _____ Objectives: Describe where DNA is located in a plant cell. Explain what procedures are required to release DNA from a plant cell Observe the extraction of Genomic DNA from plant cells. Full of DNA What can we tell about the molecular structure of DNA by studying the characteristics of DNA

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EXTRACTING DNA FROM STRAWBERRIES - Michigan

www.michigan.gov

companies. For DNA extraction, detergent is used to lyse the cell so that DNA is released into the solution. Then alcohol is added to cause the DNA to precipitate out. Strawberries are chosen for this particular experiment because strawberry cell has eight copies of the genome, and it would be easier to extract the DNA since it is

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DNA Extraction from Strawberrie - ucbiotech.org

ucbiotech.org

10 mLs DNA extraction buffer (soapy, salty wa ter) 15 mLs ice cold ethanol in test tube Procedure: Working together in groups of two, read through the entire procedure and follow the steps in Parts I, II and III. Part I - Preparing the DNA extract 1. Have one partner get the lab box while the other partner gets the strawberry and removes

  Extraction, Strawberry, Dna extraction

DNA EXTRACTION METHODS - Open University of Sri Lanka

www.ou.ac.lk

Non-Organic DNA Extraction Procedure 1. Cell Lysis Buffer - lyse cell membrane, nuclei are intact, pellet nuclei. 2. Resuspend nuclei in Protein Lysis Buffer containing a high concentration of Proteinase K. Lyse nuclear membrane and digest protein at 65oC for 2 hours. Temperature helps denature proteins, and Proteinase K auto digests itself 3.

  Extraction, Dna extraction

DNA Extraction from Strawberry - University of California ...

pbge.faculty.ucdavis.edu

Where can DNA be found in the cell? What is DNA? What are the three basic steps in any DNA extraction? What is the purpose of the detergent? Of the sodium chloride? Of the alcohol? Describe the appearance of your final product. Is this a pure DNA sample? List some practical applications where extracting DNA is necessary.

  Form, Extraction, Strawberry, Dna extraction, Dna extraction from strawberry

DNA Extraction from Strawberries - miniPCR

www.minipcr.com

miniPCR bio Learning LabsTM DNA Extraction from Strawberries Version - Release une - b Amplus C P./5 nsror’s ie For the Educator in the Room This lab extracts DNA from strawberries, as opposed to other foods, because strawberries contain large amounts of DNA. Each cell in a strawberry contains 8 copies of its genetic information

  Form, Extraction, Strawberries, Dna extraction from strawberries

DNA Reference Guide - Thermo Fisher Scientific

assets.thermofisher.com

best tor extraction of DNA from high-fat tissues such as brain, mammary tissue, or infectious samples • Columns—ideal for low- to medium-throughput processing (12–24 samples) • 96-well filter plate—ideal for high-throughput processing • High-throughput sample

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