Transcription of Quick Start Bradford Protein Assay - Bio-Rad Laboratories
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Quick Start Bradford Protein Assay - Bio-Rad Laboratories
Quick Start BradfordProtein AssayInstruction ManualFor technical service call your local Bio-Rad office, or in the US,1-800-4 BIORAD (1-800-424-6723) 9/25/2007 2:39 PM Page 1 Table of ContentsSection 1 Selecting a Protein Product Description9 Section 2 Standard Assay Microassay Protocol14 Section 3 Data Analysis18 Section 4 FAQs and Troubleshooting22 Section 5 Ordering Information26 Section 6 References28 Section 7 9/25/2007 2:39 PM Page 5 Section 1 IntroductionThe Quick Start Bradford Protein Assay is a simple and accurate procedure for determiningthe concentration of Protein in solution.
cationic form (A max = 470 nm). However, when the dye binds to protein, it is converted to a stable unprotonated blue form (A max = 595 nm) (Reisner et al. 1975, Fazekes de St. Groth et al. 1963, Sedmack and Grossberg 1977). It is this blue protein-dye form that is detected at 595 nm in the assay using a spectrophotometer or microplate reader. H +H
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