00-305/1156 acryl.poly tn - Bio-Rad Laboratories

Electrophoresistech note 1156 Paul Menter, Bio-Rad Laboratories , 2000 Alfred Nobel Drive, Hercules, CA 94547 USAI ntroductionThe unparalleled resolution and flexibility possible with polyacrylamide gel electrophoresis (PAGE) has led to its widespread use for the separation of proteins and nucleicacids. Gel porosity can be varied over a wide range to meetspecific separation requirements. Electrophoresis gels andbuffers can be chosen to provide separation on the basis ofcharge, size, or a combination of charge and key to mastering this powerful technique lies in the polymerization process itself. By understanding the importantparameters, and following a few simple guidelines, the novicecan become proficient and the experienced user can optimizeseparations even bulletin takes a practical approach to the preparation ofpolyacrylamide gels.

cause artifacts such as aberrant relative mobility, precipitation of some proteins and nucleic acids, streaking or smearing of bands, and run-to-run irreproducibility. In acrylamide, acrylic acid should be below 0.001% (w/w). This is determined by direct titration, and supported by both conductivity and pH measurement. 2.

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