PCR Optimization Student Guide 2012 - Babec

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PCR Optimization Table of Contents Fall 2012 Optimizing the Polymerase Chain Reaction Introduction ........................................ ........................................ ........................................ ..1 Review of Mathematics ........................................ ........................................ ...........................3 Solving Problems of Dilution and Concentration: Two Approaches ........................................ ..........4 Experiment Overview ........................................ ........................................ ............................7 Calculations Part 1: Overview of PCR Reaction Components ........................................ ........................8 Part 2: Varying the Magnesium Chloride Concentration ........................................ .............10 Part 3: Varying the Primer Concentration.

PCR Optimization Student Guide Fall 2012 2 ! Figure 1. An optimized PCR amplification will produce a single, bright band on a gel (as seen in the left-most lane).

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