Sanger Sequencing: Sample Preparation Guide - …

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Sanger sequencing : Sample Preparation Guide Use this as a Guide to prepare your samples for Sanger sequencing at AGRF. CONTENTS. 1 Overview ........................................ ........................................ ........................... 2. Capillary Separation (CS) or electrophoretic separation only: .............................. 2. Unpurified BDT reaction (CS+): ........................................ ................................... 2. Purified DNA (PD): ........................................ ........................................ .............. 2. Unpurified PCR product (PD+): ........................................ ................................... 3. 2 Sanger Routine sequencing Service Process: ........................................ ..... 3. 3 Three Simple Steps to using AGRF Sanger sequencing Service ............... 4. 4 References ........................................ ........................................ ....................... 4. 5 DNA Sample Preparation .

Page 2 of 13 Sanger Sequencing Sample Submission Guide (GSEQDOC00166) v1.8 Approved By: Ken McGrath Release Date: 18/11/2015. 1 Overview The Australian Genome Research Facility (AGRF) is accredited to ISO/IEC 17025:2005 in the

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