Sanger Sequencing: Sample Preparation Guide - …

Sanger sequencing : Sample Preparation Guide Use this as a Guide to prepare your samples for Sanger sequencing at AGRF. CONTENTS. 1 Overview .. 2. Capillary Separation (CS) or electrophoretic separation only: .. 2. Unpurified BDT reaction (CS+): .. 2. Purified DNA (PD): .. 2. Unpurified PCR product (PD+): .. 3. 2 Sanger Routine sequencing Service Process: .. 3. 3 Three Simple Steps to using AGRF Sanger sequencing Service .. 4. 4 References .. 4. 5 DNA Sample Preparation .. 4. Template Quality: .. 4. Template Quantity: .. 5. Primer Quality and Quantity:.. 5. DNA labelling Preparation for clients using the CS/CS+ service: .. 5. DNA Sample Preparation for clients using the PD service: .. 6. PCR Sample Preparation for clients using the PD+ service: .. 7. 6 Submission formats .. 7. Submitting Plates: .. 7. Preferred Plate Type: .. 7. Preferred Tube Type: .. 8. Sample Labelling: .. 8. Sample Presentation .. 8. Primer submission for PD+ Service: .. 8. 7 Appendix: .. 9. Protocol 1: Magnesium sulphate clean-up protocol.

Page 2 of 13 Sanger Sequencing Sample Submission Guide (GSEQDOC00166) v1.8 Approved By: Ken McGrath Release Date: 18/11/2015. 1 Overview The Australian Genome Research Facility (AGRF) is accredited to ISO/IEC 17025:2005 in the

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  Guide, Samples, Preparation, Sequencing, Sangre, Sanger sequencing, Sanger sequencing sample, Sample preparation guide

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