2 0 High Fidelity Dna Polymerase
Found 9 free book(s)Pfu DNA Polymerase - Agilent
www.agilent.com2 Pfu DNA Polymerase INTRODUCTION Pfu DNA polymerase, a proofreading DNA polymerase isolated from Pyrococcus furiosus, is an ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).1–3 These applications include cloning, gene expression, and site-directed mutagenesis.
Thermo Scientific Phusion High–Fidelity DNA Polymerase ...
assets.thermofisher.comHigh–Fidelity DNA Polymerase per 50 µL reaction volume gives good results, but the optimal amount can range from 0.5 to 2 units per 50 µL reaction depending on amplicon length and difficulty. It is not recommended to exceed 2 U/50 µL (0.04 U/µL), especially for
KOD -Plus-
bio-toyobo.cn2 mM dNTPs 1.0 ml × 1 polymerase antibodies that neutralize polymerase and 3’J5’ exonuclease activity. Quality check can be performed by amplifying the β-globin gene (3.6 Kb) and p53 gene Total Mutant KOD -Plus- 10,610 10 0.09 High fidelity PCR enzyme (A company) 10,900 68 0.62 Pfu based DNA polymerase 6,520 76 1.17
Champion pET SUMO Protein Expression System
assets.thermofisher.com2 0.01% gelatin 100 μl dNTP Mix 12.5 mM dATP ... Platinum® Taq DNA Polymerase 500 reactions 10966-034 100 units 10342-053 250 units 10342-012 Taq DNA Polymerase, Recombinant 500 units 10342-020 Platinum® Taq DNA Polymerase High 100 units 11304-011 Fidelity 500 units 11304-029 Kanamycin Sulfate 5 g 11815-024 25 g 11815-032 ...
KAPA HiFi PCR Kit Kapa/ Kit Codes and Components
rochesequencingstore.com2), 0.2 mM dATP and 1 U of Taq DNA polymerase and incubating for 5 min at 72°C. NGS library amplification NGS library amplification differs from other high-fidelity PCR applications in three noteworthy ways: (i) unlike genomic DNA or plasmids, templates are comprised of highly heterogenous populations of linear DNA or cDNA
KAPA HiFi HotStart PCR Kit
rochesequencingstore.comKAPA HiFi DNA Polymerase is supplied with two uniquely-formulated PCR buffers for optimal performance. Both buffers contain MgCl 2 at a 1X concentration of 2 mM. The Fidelity Buffer is recommended for routine high-fidelity PCR, while the GC Buffer is recommended for the amplification of GC-rich and other difficult targets. The
Polymerase Chain Reaction (PCR)
www.eeescience.utoledo.eduPolymerase Chain Reaction, 12/2004 5 MgCl 2 The concentration of MgCl 2 influences the stringency of the interaction between the primers and the template DNA. The range of MgCl 2 usually tested is from 0.5 - 4 mM in 0.5 mM increments, while …
KOD FX Neo - Toyobo
www.toyobo-global.com(2–5μL) 101– 5 cells/2μL medium or PBS μL 1–2 × 1 mm As the concentration of extracted cm 1 –2 DNA is low, 35 40 cycles are needed. × 2 mm illed rice 0.5× 0.5 mm × 1 mm On an agarose gel assay, a portion
KOD OneTM PCR Master Mix
www.toyobo-global.comcm 1 –2 DNA is low, 35 40 cycles are needed. × 2 mm illed rice 0.5× 0.5 mm × 1 mm On an agarose gel assay, a portion *In the case of the direct amplification from animal tissues, such as mouse tail, a portion of the amplification product may …