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新製品のご紹介 ヒスチジンタグ融合タヒスチジンタグ融合タ …

catalog.takara-bio.co.jp

TALON® Resin 溶出画分 Wash 123 Ni-NTA 非変性条件 溶出画分 kDa 123 83 49 32.5 SlyDa 25 Ni-NTA Resinでは、SlyDは 非特異的に吸着されてしまい、 不要なタンパク質の混入の原 因となる。 TALON® Resinでは、変性条 件、非変性条件にかかわらず 非特異的な吸着は見られない。

  Rinses, Nta resin

5.2 Protein purification - Perelman School of Medicine at ...

www.med.upenn.edu

Notes on use of Ni-NTA column: XWe recommend using new Ni-NTA resin for each purification. XUse only gravity flow for all purification steps. XPerform all steps at 4°C. XBefore mounting the luer lock syringe on top of the column, make sure that the column is filled to the top with buff er and that all air bubbles remain above the resin bed.

  Rinses, Nta resin

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