Transfection of 293F Mammalian Cells using PEI

For best transfection efficiency, cells should have a viability of >95% at the time of transfection. ... (Sigma cat. No. P4543-100G) to a final concentration of 2.2 mM. A stock solution of 220 mM VPA in water allows for the addition of 10 µl of VPA per 1 ml of final transfection volume. No further media supplementation ... Variations: High ...

  High, Efficiency, Transfection, Transfection efficiency