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www.flemingtonlab.com Total RNA isolation …
flemingtonlab.comwww.flemingtonlab.com Total RNA isolation (Guanidinium Thiocyanate-Phenol-Chloroform Extraction) Experimental Considerations 1) This method is fast and works well for preparation of total RNA.
TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
tools.thermofisher.comTotal RNA precipitate forms a white gel-like pellet at the bottom of the tube. e. Discard the supernatant with a micropipettor. 1 Precipitate the RNA a. Resuspend the pellet in 1 mL of 75% ethanol per 1 mL of TRIzol™ Reagent used for lysis. Note: The RNA can be stored in 75% ethanol for at least 1 year at –20°C, or at least 1 week at 4°C. b.