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COLUMN CHROMATOGRAPHY EXTRACTION OF PIGMENTS

users.stlcc.edu

Purpose: To separate plant pigments from spinach leaves using column chromatography. The leaves of plants contain a number of colored pigments generally falling into two categories, chlorophylls and carotenoids. Chlorophylls a and b are the pigments that make plants look green. These highly conjugated compounds capture the (non-green)

  Plants, Pigments, Plant pigments

ANALYSIS OF PLANT PIGMENTS USING PAPER …

www.chem.purdue.edu

To separate pigments from leaves of a green plant using paper chromatography and to determine the wavelength at which energy is absorbed by the individual pigments using spectrophotometry. SAFETY Goggles and aprons to be worn Petroleum ether, acetone and alcohol are volatile and flammable Avoid breathing vapors of the reagents

  Plants, Pigments, Plant pigments

LAB #6 Photosynthesis and Cellular Respiration

www.lamission.edu

The various plant pigments have differing degrees of hydrophobicity. Therefore, if we use a liquid solvent that is hydrophobic, different plant pigments will move at differing rates through the piece of paper as the liquid solvent is absorbed upward. In this way, individual pigments can be separated into bands on the filter paper.

  Plants, Pigments, Plant pigments

LAB 7 Photosynthesis

www.lamission.edu

The various plant pigments have differing degrees of hydrophobicity. Therefore, if we use a liquid solvent that is hydrophobic, different plant pigments will move at differing rates through the piece of paper as the liquid solvent is absorbed upward. In this way, individual pigments can be separated into bands on the filter paper.

  Plants, Pigments, Plant pigments

Experiment 6 — Thin-Layer Chromatography

www.amherst.edu

Experiment A. Plant pigments. Plants use a number of different pigments in their light-harvesting systems. These compounds belong to the chlorophyll and carotenoid classes. Representative members of these groups, chlorophyll (a and b) and β-carotene are shown below. N Mg N N N R O O OCH3 Chlorophyll a: R = CH3 Chlorophyll b: R = CH=O O O ...

  Plants, Pigments, Plant pigments

Analysis of -Carotene in Carrot by Spectrophotometry

www.pharmacy.mahidol.ac.th

INTRODUCTION The term “carotenoids” refers to a family of more than 600 different plant pigments, which are responsible for many colors (red, orange and yellow etc.) of

  Plants, Pigments, Plant pigments

Plant Pigment Chromatography

vaei.vai.org

Plant pigments are colored molecules that absorb light at specific wavelengths. These molecules capture the energy of sunlight and use it to make their own food. For more information about the different types of plant pigments, provide this resource (or your own) for students to review: Photosynthetic Pigments ...

  Plants, Pigments, Plant pigments

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