Search results with tag "Acetone precipitation of proteins"
Acetone precipitation of proteins - rtsf.natsci.msu.edu
www.rtsf.natsci.msu.eduAcetone precipitation of proteins Adapted from Pierce TR0049.0 www.piercenet.com Pre-cool a bottle of Acetone in ice or in a 4C cooler prior to start of the procedure 1. Working on wet ice, aliquot the needed volume of your protein solution into a new tube. 2. To this, add 4 Volumes of cold acetone.
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com7. Allow the acetone to evaporate from the uncapped tube at room temperature for 30 minutes. Do not over-dry pellet, or it may not dissolve properly. 8. Add buffer appropriate for the downstream process and vortex thoroughly to dissolve protein pellet. TR0049.1 Acetone precipitation of proteins
Acetone precipitation of proteins - bidmcmassspec
www.bidmcmassspec.org• Precipitation has an advantage over dialysis or desalting methods in that it enables concentration of the protein sample as well as purification from undesirable substances. • One disadvantage of protein precipitation is that proteins may be denatured, making the pellet difficult to re-solubilize.
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com7. Allow the acetone to evaporate from the uncapped tube at room temperature for 30 minutes. Do not over-dry pellet, or it may not dissolve properly. 8. Add buffer appropriate for the downstream process and vortex thoroughly to dissolve protein pellet. TR0049.1 Acetone precipitation of proteins
Acetone Precipitation Proteins - University of Arizona
proteomics.arizona.eduArizona Proteomics Consortium Protocol 09/18/09 Acetone Precipitation of Proteins Equipment acetone-tolerant polypropylene tubes table top centrifuge
Acetone precipitation of protein - Fred Hutch
research.fhcrc.orgAcetone precipitation of protein Jeff Ranish, Insitute of Systems Biology June 2007 This procedure is suitable for recovering proteins from most aqueous