Transcription of 1-800-4BIORAD Protein Assay Bio-Rad
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LIT33C 8/31/98 02:25 PM Page a Bio-Rad Protein Assay For Technical Service Call Your Local Bio-Rad Office or in the Call 1-800-4 BIORAD. (1-800-424-6723). LIT33C 8/31/98 02:25 PM Page b Table of Contents Section 1 1. Principle .. 1. Product 3. Materials Required but Not 3. Section 2 4. Reconstituting the Standard .. 4. Standard Procedure .. 4. Microassay Procedure .. 5. Microtiter Plate Protocols .. 6. Section 3 Common 8. Section 4 Troubleshooting Guide .. 12. Section 5 Ordering 13. Section 6 Safety Information .. 14. Section 7 References .. 24. LIT33C 8/31/98 02:25 PM Page 1. Section 1. Introduction The Bio-Rad Protein Assay , based on the method of Bradford, is a simple and accurate procedure for determining concentration of solubi- lized Protein . It involves the addition of an acidic dye to Protein solution, and subsequent measurement at 595 nm with a spectrophotometer or microplate reader. Comparison to a standard curve provides a relative measurement of Protein concentration. Principle The Bio-Rad Protein Assay is a dye-binding Assay in which a differ- ential color change of a dye occurs in response to various concentrations of Protein .
Bio-Rad Protein Assay For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723) LIT33C 8/31/98 02:25 PM Page a
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