Transcription of 1-800-4BIORAD Protein Assay Bio-Rad
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1-800-4BIORAD Protein Assay Bio-Rad
Bio-RadProtein AssayFor Technical ServiceCall Your Local Bio-Rad Office orin the Call 1-800-4 BIORAD(1-800-424-6723)LIT33C 8/31/98 02:25 PM Page aTable of ContentsSection 1 Introduction .. Required but Not 2 Instructions .. Reconstituting the Standard .. Procedure .. Procedure .. Plate Protocols ..6 Section 3 Common Questions .. 8 Section 4 Troubleshooting Guide .. 12 Section 5 Ordering 13 Section 6 Safety Information .. 14 Section 7 References .. 24 LIT33C 8/31/98 02:25 PM Page b1 Section 1 IntroductionThe Bio-Rad Protein Assay , based on the method of Bradford, is asimple and accurate procedure for determining concentration of solubi-lized Protein . It involves the addition of an acidic dye to Protein solution,and subsequent measurement at 595 nm with a spectrophotometer ormicroplate reader.
assays using the standard assay procedure, or 2,250 assays using the microassay procedure. The Dye Reagent Concentrate can be purchased in a kit with one of two standards: Bovine gamma globulin (Kit I, catalog number 500-0001) or bovine serum albumin (Kit II, catalog number 500-0002). The Bio-Rad Protein Assay is for research use only.
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