Transcription of 1 Basic Principles of Fluorescence Spectroscopy
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1 Basic Principles of Fluorescence Spectroscopy
1 Basic Principles of Fluorescence and Emission of LightAsfluorophores play the central role influorescence Spectroscopy and imaging wewill start with an investigation of their manifold interactions with light. Afluorophoreis a component that causes a molecule to absorb energy of a specific wavelength andthen re-remit energy at a different but equally specific wavelength. The amount andwavelength of the emitted energy depend on both thefluorophore and the chemicalenvironment of thefluorophore. Fluorophores are also denoted as chromophores,historically speaking the part or moiety of a molecule responsible for its color. Inaddition, the denotation chromophore implies that the molecule absorbs light whilefluorophore means that the molecule, likewise,emits light.
rays X- rays IR Microwave Radio Visible UV Figure 1.1 The electromagnetic spectrum. 2j ... C¼C C¼C C¼C framework, they spread out, minimizing repulsion between them), then one can view the energetics of this system as arising from the simple ... r and E n ¼ n2 h2 8mL2 where n is the quantum number (n¼1, 2, 3,...) giving the number of ...
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