Transcription of 1 Buffer Preparation - MD Anderson Cancer Center
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1 Buffer Preparation - MD Anderson Cancer Center
Buffer Preparation (Shi Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 2 150-155 pH 2 120-125 pH 2 80-85 Autoclavable. 2. EDTA M ( ) , 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris, 50 mM EDTA) 4 L 968 g Tris ml glacial acetic acid 400 ml M EDTA To make 1x TAE 20 L, add 400 ml 50X Buffer into L ddH2O.
5. 5X SDS Loading Sample Buffer 100 ml Stock solution Add volume 250 mM TrisHCl pH6.8 1 M 25 ml 10% SDS 10 g 30% Glycerol 30 ml 5% β-mercapitalethanol (or 0.5M DTT) 5 ml 0.02% bromophenol blue 1% 2 ml 6. 6X DNA loading sample buffer: (40% sucrose, 0.01-0.02% BPB) 100 …
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