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Search results with tag "Sample loading buffer"
6X SDS Protein Loading Buffer - Morganville Scientific
morganvillesci.comAug 06, 2016 · 1. Mix well and dissolve any precipitates in the sample loading buffer by incubating at 37°C. 2. Add 9 µL β-mercaptoethanol to 91 µL 6X SDS Protein Loading Buffer and mix well. Make a 1:5 dilution of 6X SDS protein loading buffer (containing the reducing agent) to protein sample. For example, add 1 µL 6X SDS protein loading buffer to 5 µL ...
1 Buffer Preparation - MD Anderson Cancer Center
www.mdanderson.org5. 5X SDS Loading Sample Buffer 100 ml Stock solution Add volume 250 mM TrisHCl pH6.8 1 M 25 ml 10% SDS 10 g 30% Glycerol 30 ml 5% β-mercapitalethanol (or 0.5M DTT) 5 ml 0.02% bromophenol blue 1% 2 ml 6. 6X DNA loading sample buffer: (40% sucrose, 0.01-0.02% BPB) 100 …