1. Plasmid structure 2. Plasmid replication and copy ...
Plasmid replication requires host DNA replication machinery. 2. Most wild plasmids carry genes needed for transfer and copy number ... Large or small region of homologous DNA cloned that will integrate into the chromosomal target. 5. Need a counter selection method to kill the donor cells 6. Screen for what you think is correct.
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Lecture 10: Multiple Testing - UW Genome Sciences
www.gs.washington.eduWhy Multiple Testing Matters Genomics = Lots of Data = Lots of Hypothesis Tests A typical microarray experiment might result in performing 10000 separate hypothesis tests.
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DNA Isolation from Strawberries
www.gs.washington.edusource for extracting DNA because they are easy to pulverize and contain enzymes called pectinases and cellulases that help to break down cell walls. And most important, strawberries have eight copies of each chromosome (they are octoploid), so there is a lot of DNA to isolate. The purpose of each ingredient in the procedure is as follows:
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Lecture 10: Multiple Testing
www.gs.washington.eduFalse Discovery Rate m 0 m-m 0 m V S R Called Significant U T m - R Not Called Significant True True Total Null Alternative V = # Type I errors [false positives] •False discovery rate (FDR) is designed to control the proportion of false positives among the set of rejected hypotheses (R)
Lecture 7: Hypothesis Testing and ANOVA
www.gs.washington.edu•Calculate a test statistic in the sample data that is ... candidate gene. We then divide these N individuals into ... Sum of MS F Squares Source of df Variation! SST G k"1! SST E N"k! SST G k"1 SST E N"k. Non-Parametric Alternative • Kruskal-Wallis …
Lecture 4: Random Variables and Distributions
www.gs.washington.edu•Before data is collected, we regard observations as random variables (X 1,X 2,…,X n) •This implies that until data is collected, any function (statistic) of the observations (mean, sd, etc.) is also a random variable •Thus, any statistic, because it is a random variable, has a probability distribution - referred to as a sampling ...
I-9 Form: Instructions for Nonresident on H-1B or TN Visa
www.gs.washington.eduI-9 Form: Instructions for Nonresident on H-1B or TN visa Instructions for both New Hires and Updating & Reverification For more detailed information about completing Form I-9, employers and employees should refer to the Handbook for Employers: Instructions for Completing Form I-9 (M-274). _____
Lecture 5: Estimation - University of Washington
www.gs.washington.eduBayesian Estimation: ÒSimpleÓ Example ¥I want to estimate the recombination fraction between locus A and B from 5 heterozygous (AaBb) parents. I examine 30 gametes for each and observe 4, 3, 5, 6, and 7 recombinant gametes in the Þve parents. What is the mle of the recombination fraction? ¥Tedious to show Bayesian analysis. LetÕs simplify ...
Lecture 9: Linear Regression - University of Washington
www.gs.washington.eduLecture 9: Linear Regression. Goals • Linear regression in R •Estimating parameters and hypothesis testing ... •Previous coding would result in colinearity •Solution is to set up a series of dummy variable. In general for k levels you need k-1 dummy variables x 1 …
Lecture 2: Descriptive Statistics and Exploratory Data ...
www.gs.washington.eduMultivariate Data •Organize units into clusters •Descriptive, not inferential •Many approaches •“Clusters” always produced Clustering Data Reduction Approaches (PCA) •Reduce n-dimensional dataset into much smaller number •Finds a new (smaller) set of variables that retains most of the information in the total sample
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DNA replication in 7 easy steps - BIOLOGY FOR LIFE
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DNA, RNA, replication, translation, and transcription ...
sites.engineering.ucsb.eduOct 27, 2010 · Replication forks form and involve a leading and a lagging strand • DNA is directional; two strands are antiparallel ... from the region coding for the protein TATA sequence or TATA box 4. Other proteins assemble to form a large transcription complex 5. Chromatin-remodeling proteins are involved to make DNA accessible from the wound
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www.citiid.cam.ac.ukexposed populations and/or increased replication. Data on replication are limited however. Delta spike was previously shown to confer more efficient cell-cell fusion kinetics compared to Wuhan-12, and syncytia formation has previously been associated with pathogenesis3. Moreover, changes in the PBCS have been associated with pathogenicity.
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assets.publishing.service.gov.ukOmicron S gene at position 214 and a primer-probe set for the same region in the Delta S gene which lacks this insertion, allowing for the proportion of each variant in the same sample to be determined. We first validated the specific RT-qPCR by creating a series of artificial mixes of RNA from each variant (left hand graph, Figure 3).
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engineering.tufts.eduParylene exhibits very little absorption in the visible region and is, therefore, transparent and colorless. Below about 280 nm both Parylene N and C absorb strongly, as shown in Figure 4. Figure 4. Ultraviolet Absorption Spectra of Parylene N and C The infrared spectra for 1 mil films are shown in Figure 5, Figure 6, and Figure 7.