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ÄKTAdesign Purification - Harvard University

KTA KTAdesign PurificationMethod handbookGE HealthcareProtocol FinderPlanning protein purificationStandard Purification protocol for proteins .. 28 Adjustment and optimization .. 33 Planning peptide Purification synthetic peptidesStandard Purification protocol for synthetic peptides .. 47 Adjustment and optimization .. 54 Peptides from natural sourcesStandard Purification protocols for natural peptides .. 63 Adjustment and optimization .. 69 Protein fragmentsStandard Purification protocols for protein fragments .. 77 Adjustment and optimization .. 80 Planning oligonucleotide purificationStandard Purification protocol for synthetic phosphorothioate oligonucleotides .. 87 Anion exchange chromatography protocol .. 94 RPc protocol .. 95 Adjustment and optimization .. 96 Changing the scale .. 99 Trouble-shooting .. 101 Column maintenance .. 103 The MapNaturalSourcePureProteinPurePeptideFr agmentsQuantitativeAnalysisAmino AcidAnalysissPeptideMappingPeptideLibrar yPeptideSynthesisSequencingScreeningModi ficationsOptimization CycleActiveAnaloguesClonedGeneDNAS ynthesisSequencingProbeAntisensePrimersI solatedGeneDiagnosticSequencingor PCRA nalyticalDNA electro -phoresisGeneFishingNaturalSourceProtein /PeptideStructureGeneStructurePage 8 Gene fishingloopPage 61 Page 77 Page 77 Page 43 Page 21 Page 87 Page 87 Page 87 Page 87 KTAdesign Purification User Manual 8- 24-23

ÄKTAdesign Purification – User Manual 8- 24-23 Edition AD Contents 4 Appendix I – Tips and hints.....125 I. Sample stability.....

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Phosphorylation Analysis by Mass Spectrometry

kirschner.med.harvard.edu

why a phosphorylation analysis using LC/MS failed. To test this we used a set of peptide/phosphopeptide pairs that var-ied in length from 7 to 17 amino acids, resembling the size of peptides commonly observed in tryptic digests. Cysteine and methionine-containing peptides were not included in this

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User Guide - Harvard University

kirschner.med.harvard.edu

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kirschner.med.harvard.edu

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kirschner.med.harvard.edu

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kirschner.med.harvard.edu

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kirschner.med.harvard.edu

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