Transcription of Phosphorylation Analysis by Mass Spectrometry
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Phosphorylation Analysis by Mass Spectrometry
Research Phosphorylation Analysis by mass Spectrometry MYTHS, FACTS, AND THE CONSEQUENCES FOR QUALITATIVE AND QUANTITATIVE MEASUREMENTS*. Hanno Steen , Judith A. Jebanathirajah **, John Rush , Nicolas Morrice , and Marc W. Kirschner . The mass spectrometric Analysis of protein phosphoryla- ever, this number is in stark contrast to the actual number of tion is still far from being routine, and the outcomes Phosphorylation sites found so far. For instance, the Phos- thereof are often unsatisfying. Apart from the inherent database ( ) currently lists 1703. problem of substoichiometric Phosphorylation , three ar- experimentally verified Phosphorylation sites for 556 different guments as to why Phosphorylation Analysis is so prob- proteins derived from eukaryotes, the human protein refer- lematic are often quoted, including (a) increased hy
why a phosphorylation analysis using LC/MS failed. To test this we used a set of peptide/phosphopeptide pairs that var-ied in length from 7 to 17 amino acids, resembling the size of peptides commonly observed in tryptic digests. Cysteine and methionine-containing peptides were not included in this
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