Transcription of 260/280 and 260/230 Ratios - dna.uga.edu
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260/280 and 260/230 Ratios - dna.uga.edu
T042 TECHNICAL BULLETIN NanoDrop Spectrophotometers Thermo Fisher Scientific - NanoDrop products Wilmington, Delaware USA Technical support: 302-479-7707 260/280 and 260/230 Ratios C Introduction Absorbance measurements made on a spectrophotometer, including any Thermo Scientific NanoDrop Spectrophotometer, will in-clude the absorbance of all molecules in the sample that absorb at the wavelength of interest. Since nucleotides, RNA, ssDNA, and dsDNA all absorb at 260 nm, they will contribute to the total absorbance of the sample.
using a NanoDrop™ Spectrophotometer, nucleic acid samples will require purification prior to measurement. 260/280 Ratio The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA. A ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA.
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