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2D Correlation Experiments: HSQC, HMQC, HMBC,

2D Correlation Experiments: HSQC, HMQC, HMBC, BCMB/CHEM 8190 Two Dimensional NMR Spectroscopy (ppm) intensity intensity (f2) (f1) Two-dimensional (2D) NMR spectra are presented along two orthogonal axes (rather than one for 1D NMR) - typically, the two axes are chemical shifts ( Correlation spectroscopy), but not limited to this ( J-coupling, etc.) - convention is typically for directly observed dimension to be presented along the x-axis - correlations between x- and y-axis variables based on: - J coupling (COSY, TOCSY, HSQC) - dipolar interactions (NOESY) - chemical exchange (EXSY) Two Dimensional NMR Spectroscopy A general scheme for 2D experiments describes these as constructed from 'preparation', 'evolution' and 'mixing' elements - preparation: create magnetization of interest - evolution 1: increment t1 time period, evolve

(biomolecular) NMR - the HSQC experiment is one of the fundamental building blocks of scores of multidimensional, heteronuclear and triple resonance NMR experiments - the 1H-15N pairs in amide groups of amino acids in proteins are convenient reporters for each amino acid - the 1H, 15N-HSQC spectrum of a protein is a

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