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FTIR Analysis of Protein Structure

FTIR Analysis of Protein StructureWarren to Protein structureThe first structures of proteins at an atomic resolution were determined in the late 1950 From that time to the early 1990 s approximately 300 Protein structures were added to the list, primarily using X-ray crystallography. Currently over 20,000 structures are solved,2 some of these using the newer technique of multidimensional nuclear magnetic resonance spectroscopy (NMR). While X-ray crystallography and NMR spectroscopy provide the greatest level of detail about a Protein s Structure , there are many situations where these techniques cannot be applied. Other techniques, such as FTIR, may not provide the same level of structural detail, but can be applied readily to further our understanding of how proteins functionBefore describing the structural information that FTIR spectroscopy provides, a brief introduction to Protein Structure is given.

B. Obtaining structural information about proteins using FTIR spectroscopy FTIR spectroscopy provides information about the secondary structure content of proteins, unlike X-ray crystallography and NMR spectroscopy which provide information about the tertiary structure. FTIR spectroscopy works by shining infrared radiation on a

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