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3. Troubleshooting for Increased Pressure

191I. HPLC ColumnsII. uhplc ColumnsIV. Related ProductsV. ApplicationsVI. Technical NotesVII. Index III. Preparative Packing Materials3. Troubleshooting for Increased PressureIntroductionRepeated analysis may increase back Pressure . Continuous use of HPLC columns under high Pressure can cause deteri-oration and overload of the equipment. Therefore, it is important to monitor column back Pressure regularly and solve the problem of the Clogging SiteThe back Pressure increase can be due to clogging of a column or clogging of the equipment. First of all, identify theclogging the system components one by one to identify the clogged component(s). Start by disconnecting the column from the system and measure the Pressure of flowing mobile phase.

192 I. HPLC Columns II. UHPLC Columns III. Preparative Packing Materials IV. Related Products V. Applications VI. Technical Notes VII. Index What should I do when a …

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