Transcription of 4. 荧光偏振 - Shandong University
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4. 荧光偏振 - Shandong University
4.. If linear polarized light is used to excite an ensemble of fluorophores, only those fluorophores aligned with the plane of polarization will be excited. There are 2 scenarios for the emission. Perrin Weber Dandliker Jolley 1.. P A .. The fluorescence anisotropy of a fluorophore reflects the molecule's ability to rotate in its microenvironment, which depends on the viscosity of the solution, the fluorescence lifetime of the fluorophore, and the size and mass of the molecule to which the fluorophore is attached. I//-I I//-I . P= A=. I//+I I//+2I P = 3 A / (2 + A). Fluorescence polarization --p A = 2 P / (3 P). 2. Fluorescence anisotropy A or r (1) . A I//=I P=0 .. B I// I 0 P= 1 .. C I// I 0 0<P<1 .. 3. Fluorescence polarization (FP) can be considered a competition between the molecular motion and the lifetime of fluorophores in solution.
viscosity of the solution, the fluorescence lifetime of the fluorophore, ... mobility, myosin reorientation and protein–protein interactions at the molecular level. ... protein or complex, it is possible for emission light energy from A to be absorbed by B and to be emitted as part of B’s emission spectrum. A/Q.
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