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7. Thin-Layer Chromatography - Chemistry Courses

937. Thin-Layer ChromatographyYou will need to bring your package of TLC plates on this day. Not all plates will be used inthis experiment; some will be needed in lab the following week. Be sure to include all theanalgesics and solvents used in this experiment in your Chemical Data Tables. Don t forget toinclude the Chromatographic Behavior Comparison section in this PreLab. An example can befound in section exercise:Using a greatly magnified diagram of a TLC plate, locate or define each of the following terms:1. Origin2. Stationary Phase3. Mobile Phase4. Solvent Front5. Component spot 6. Rf value7. Development8. Visualization of spots9. What is meant by differential partitioning between stationary and mobile phase?10. What is the recommended procedure for cleaning a TLC spotting capillary?IntroductionChromatography is the separation of two or more compounds or ions by the distributionbetween two phases, one which is moving and the other which is stationary.

93 7. Thin-Layer Chromatography You will need to bring your package of TLC plates on this day. Not all plates will be used in this experiment; …

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