Transcription of 9221 MULTIPLE-TUBE FERMENTATION TECHNIQUE FOR …
{{id}} {{{paragraph}}}
Example: biology
9221 MULTIPLE-TUBE FERMENTATION TECHNIQUE FOR …
9221 MULTIPLE-TUBE FERMENTATION TECHNIQUE FOR MEMBERS OF THECOLIFORM GROUP*9221 A. IntroductionThe coliform group consists of several genera of bacteriabelonging to the family Enterobacteriaceae. The historical defi-nition of this group has been based on the method used fordetection, lactose FERMENTATION , rather than on the tenets ofsystematic bacteriology. Accordingly, when the fermentationtechnique is used, this group is defined as all facultative anaer-obic, gram-negative, non-spore-forming, rod-shaped bacteriathat ferment lactose with gas and acid formation within 48 h at35 standard test for the coliform group may be carried out bythe MULTIPLE-TUBE FERMENTATION TECHNIQUE or presence absenceprocedure (through the presumptive-confirmed phases or com-pleted test) described herein, the membrane filter (MF) TECHNIQUE (Section 9222) or the enzymatic substrate coliform test (Section9223).
Incubate the inoculated brilliant green lactose bile broth tubes at 35 0.5°C. Formation of gas in any amount in the inverted vial of the brilliant green lactose bile broth fermentation tube at any time within 48 o3 h constitutes a positive confirmed phase. To estimate the coliform density, calculate the MPN value from
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document: