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Chapter 6: Microbial Nutrition and Growth

Chapter 6: Microbial Nutrition and GrowthMicrobial Growth : Refers to an increase in cellnumber, not in cell size. Bacteria grow and divide by binaryfission, a rapid and relatively simple for GrowthPhysical : Microbes are looselyclassified into several groups based on their preferred temperature : Cold-loving . Can grow at 0oC. Two groups: True Psychrophiles: Sensitive to temperatures over 20oC. Optimum Growth at 15oC or below. Found in very cold environments (North pole, ocean depths). Seldom cause disease or food spoilage. Psychrotrophs: Optimum Growth at 20 to 30oC. Responsible for most low temperature food for GrowthPhysical : Middle loving . Most bacteria. Include most pathogens and common spoilage organisms. Best Growth between 25 to 40oC.

Brilliant Green Agar: Green dye selectively inhibits gram-positive bacteria. Used to isolate ... •Bile salts and crystal violet discourage growth of gram-positive bacteria. •Lactose plus pH indicator: Lactose fermenters produce pink or red colonies, nonfermenters are colorless.

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